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Neuro & cognitive

PE-22-28

A rodent-stage antidepressant candidate being discussed online as a nootropic peptide.

L2Animal / lab studiesNot approved for this usePreclinical
How settled this rating is:
very-low confidence in the current evidence rating
Routes studied:
Not established · Subcutaneous injection, Nasal (marketed but never approved)
Also known as:
spadin analogue, TREK-1 blocker peptide
Last reviewed:
2026-09-06
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Evidence passport

Last reviewed 2026-09-06

A one-screen summary of what is known about this compound. Every field describes the evidence and legal status — not how safe or effective it is, and not a recommendation. The 0-100 figures are evidence-strength ratings, not percentages.

Evidence level (type of study, not a quality rating)
L2Animal / lab studies
How settled this rating is
Very low confidence in rating
Human efficacy evidence
5 of 100 evidence strength
Human safety evidence
5 of 100 evidence strength
Regulatory status
Not approved for this use
Development
Preclinical
Routes
SC injection· unapprovedNasal· unapproved
Evidence base
Mostly animal and lab studies
Furthest stage
Preclinical
Source curation
Sources fully curated
How far human research has progressed
Preclinical (rodent) on our curated record; no verified completed human trial

Most of this evidence comes from animals and cells

The supporting research for PE-22-28 is predominantly preclinical (Level 1–2). That is a legitimate starting point for science and a poor basis for personal decisions. Human results are not simply a scaled-up version of mouse results.

Overview

Human efficacy evidence strength5 of 100 evidence strength

Higher = more and better-quality human evidence. It does not mean the effect is bigger or that the compound works for you.

Preclinical (animal / lab) evidence strength45 of 100 evidence strength

Higher = more animal, tissue or cell research. Preclinical results are a starting point, not proof of a human benefit.

Human safety evidence strength5 of 100 evidence strength

Higher = more mature, better-documented human safety data. It does not mean the compound is safer than one with a lower number.

How to read these scores

Every number here rates the evidence, not the compound. They are editorial 0-100 strength ratings, not percentages, and not a rating out of five stars.

  • Human efficacy evidence strengthHigher = more and better-quality human evidence. It does not mean the effect is bigger or that the compound works for you.
  • Preclinical evidence strengthHigher = more animal, tissue or cell research. Preclinical results are a starting point, not proof of a human benefit.
  • Human safety evidence strengthHigher = more mature, better-documented human safety data. It does not mean the compound is safer than one with a lower number. A score of 80 does not mean “80% safe”.
  • Evidence level (L0-L5) — the strongest type of study behind the compound, from anecdote (L0) to large replicated human trials (L5). It is a description of the study evidence, not a quality, safety or recommendation rating.
  • Confidence — how likely we think it is that this rating changes as new research lands. Low confidence means the picture is unsettled, not that the compound is bad.

None of these ratings is medical advice, a safety guarantee, or a recommendation to use anything. How we grade evidence.

How far human research has progressed: Preclinical (rodent) on our curated record; no verified completed human trial

Routes & formulations

Marketed / research routes — never approved

SC injection· unapprovedNasal· unapproved

Marketed as injectable and nasal preparations. Neither is an approved formulation, and animal-study routes do not validate marketed ones.

Route availability is not evidence of efficacy, and evidence from one route does not transfer to another. The Registry never provides dosing or sourcing information.

Peptide lifecycle

  1. Discovery

  2. Preclinical

    Furthest established stage

  3. Phase I

  4. Phase II

  5. Phase III

  6. Approved

Preclinical

Our curated record establishes rodent work only. If a human trial exists, we have not verified it — and we will not describe one that we cannot source.

What it is

A short peptide derived from spadin, developed in academic laboratories to block the TREK-1 potassium channel — a target linked to antidepressant response in rodents.

What people claim

  • Fast-acting antidepressant effect
  • Neurogenesis and cognitive enhancement
  • Safer than conventional antidepressants

What the evidence actually says

The rodent literature is genuinely interesting: TREK-1 blockade produces antidepressant-like behaviour and neurogenesis markers in mice. That is where the evidence stops. There is no verified human trial in our record, which means every claim about mood, cognition or safety in people is extrapolation from mice. Novel target, zero human answers.

Useful next reads based on this compound — not recommendations.

Human evidence

  • No completed human trial is verified in our curated record.
  • Reports of benefit come from anecdote and marketing, which are not evidence.

Preclinical evidence

  • Rodent studies of TREK-1 blockade reporting antidepressant-like behavioural effects and hippocampal neurogenesis markers.

Animal and cell findings are Level 1–2 evidence.

Mechanism and pathways

  • Blockade of the TREK-1 two-pore potassium channel, a target implicated in rodent antidepressant response.
  • Downstream effects on serotonergic signalling and neurogenesis reported in animals.

Safety and unknowns

  • There is no human safety dataset at all.
  • Nasal and injectable research-market products carry purity and sterility risks independent of the molecule.

Cancer relevance

This section covers questions raised by how the compound works in the body, plus anything regulators and trial teams keep an eye on. It is not a statement that this compound causes cancer — for most compounds here, no human data exist in either direction.

No established human signal

No cancer signal established. Human data are absent rather than reassuring.

Regulatory status

Not an approved medicine and not, on our curated record, established in any completed human trial. It is sold as a research chemical and discussed in nootropic and depression communities.

Evidence grade

L2Animal / lab studiesVery low confidence in rating

Studies in animals or tissue, with no dependable human results yet. Mice are not tiny humans; promising animal data often fails in people.

What would change our rating?

  • A registered, completed human trial with published results.
  • Any human pharmacokinetic or safety data at all.

References and sources

Sources fully curated

Source hierarchy: regulatory documents and trial registries first, peer-reviewed work next, sponsor material flagged as non-independent. We never use supplier or clinic marketing as evidence, and where a verified source has not yet been curated we say so rather than inventing one.

Evidence change history

These entries record changes to our assessment of the published evidence — not changes to the compound itself.

No recorded changes yet. Baseline position set on 2026-09-06.